Modification of proteins by addition of K63-linked polyubiquitin chains is implicated in a variety of cellular events, including DNA repair, signal transduction and receptor endocytosis.
A monoclonal antibody (mAb) has been generated that specifically recognizes K63-linked polyubiquitin chains, but not any other isopeptide-linked (K6, K11, K27, K29, K33, or K48) polyubiquitin chain or monoubiquitin. The specificity of this K63-linked ubiquitinspecific monoclonal antibody was demonstrated using di-ubiquitin chains and with endogenously ubiquitinylated species in both cell lysates and intact cells.
The antibody has been extensively characterised by ELISA, one-dimensional Western blotting, immunofluorescence utilising cells transfected with wild type and [K63R]ubiquitin, and immunohistochemistry using material derived from the brains of humans suffering from a variety of neurodegenerative disease.
应用类型
免疫原
Synthetic peptide containing the ubiquitin K63-isopeptide motif