WB; IHC, Frozen; IC; Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) and Flow Cytometry (2 ug per 10^6 cells). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:100 - 1:500 is recommended for IC and IH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A preparation of enzymatically dephosphorylated pig neurofilaments including NF-L, NF-M and NF-H. Screening was by ELISA on the immungen followed by immunofluorescence microscopy. The epitope for this antibody is not in the N-terminus but its exact location is not known. It is thought to be somewhere within the central alpha helical "rod" region of the molecule.